It looks definitely German, and seems to be close to the works of Barthel Bruyn the Elder (1493-1555) or the Younger (ca. 1530-1610). They often portrayed similar women with elaborate headdresses, large jewels, and fine hands with flowers in them.
Most Bruyn portraits of woman (and most woman portraits from the period in general) look to the left, as they were the rightside panel of either a diptych (with the portrait of their husband) or a triptych (with the husband as the left wing, and central some religious scene). A woman looking right is probably some unmarried woman (e.g. a widow).
Jan Mostaert, ca. 1515-1520
Master of Frankfurt, detail of Saint Catherine
Joos van Cleve
Better possibilities seem to be some contemporaries like the Master of Frankfurt (1460-1533), Jan Mostaert (1475-1555) or even at a stretch Joos van Cleve (1485-1540), who is probably a bit too late for this.
In summary: this work is probably Flemish, first quarter of the 16th century. Artist and sitter are unknown, but the position of the woman, facing right instead of left, is unusual and may point to some form of independent status. The work has had some damage (overcleaning), but even so the estimate is way too low and even as an anonymous work this should be closer to 5,000 Euro.